FISH Chromosomes

FISH (fluorescence in situ hybridization) is a cytogenetic technique developed by biomedical researchers in the early 1980s that is used to detect and localize the presence or absence of specific DNA sequences on chromosomes.  FISH uses fluorescent probes that bind to only those parts of the chromosome with which they show a high degree of sequence complementarity.  Fluorescence microscopy can be used to find out where the fluorescent probe bound to the chromosomes.  FISH is often used for finding specific features in DNA for use in genetic counselling, medicine, and species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples.  In this context, it can help define the spatial-temporal patterns of gene expression within cells and tissues.

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