Ready, Set, Replicate

Although biologists have been watching cells divide under the microscope for ~150 years, visualizing the steps of interphase in live cells has been difficult. In 2008 Sakaue-Sawano et al. developed a clever method, called FUCCI imaging, to watch a critical decision during interphase – the choice to start replicating DNA. (reblogged from

Ready, Set, Replicate (Teresa Davoli, Eros Lazzerini Denchi, Titia de Lange) Youtube link

In FUCCI imaging, red and green fluorescent proteins are fused to two interphase regulators, Cdt1 and Geminin, respectively. Cdt1 exists only during G1 and early S phase, whereas Geminin exists during S/G2. Thus, cells appear red in G1, yellow in early S, and green in late S and G2 phases. Here human fibroblasts are visualized by time-lapse live-cell imaging (phase-contrast and fluorescent images acquired every 15 min with 10X objective). (Reblogged from

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